Role of pollen morphology in taxonomy and detection of adulterations in crud drugs

Pollen characters are useful in solving complicated problems of interrelationships between various taxa and assessment of their status in the classi ication, particularly with reference to the families, subfamilies, tribes, genera, species, and subspecies. Pollen morphology is conducted as an aid to the morphological study and a signi icant tool for modern taxonomist for the delimitation of species. Mature pollen grain size, exine sculpturing, and number of pores are the most distinctive features. Palynological data has been useful at generic and speci ic level. This analysis also helps in qualitative analysis of drug powder and the correct identi ication of drug. It plays an important role in our daily life as well. Aerobiology has received much attention due to its wide application in allergology, forestry, agriculture, horticulture, archaeology, and plant geography. Pollen morphological characters were fully appreciated by authors such as Lindley, von Mohl & Fritzsche as early as 1830-1840. But generally, these characters are overlooked by taxonomist due to lack of suf icient data. Recently, however, considerable attention is being paid to pollen morphology, and the science of palynology (study of spores & pollen grains) has entered into current taxonomic thinking.


Introduction
The use of herbal medicine for the treatment of diseases and infections is a safe and traditional therapy.
Pollen morphological characters are used to identify adulterations in crud drugs or dry herbs of some medicinal plants as pollen grains are very minute and are adhered in any part of the crud drugs.
In developing countries, medicinal plants are attaining greater importance in the primary health care of individuals and communities. Medicinal plants form a large group of economically important plants that provide the basic raw materials for indigenous pharmaceuticals Pollen characters are useful in solving complicated problems of interrelationships between various taxa and assessment of their status in the classi ication, particularly with reference to the families, subfamilies, tribes, genera, species, and subspecies. Pollen morphology is conducted as an aid to the morphological study and a signi icant tool for modern taxonomist for the delimitation of species. Mature pollen grain size, exine sculpturing, and number of pores are the most distinctive features. Palynological data has been useful at generic and speci ic level. This analysis also helps in qualitative analysis of drug powder and the correct identi ication of drug. It plays an important role in our daily life as well. Aerobiology has received much attention due to its wide application in allergology, forestry, agriculture, horticulture, archaeology, and plant geography.
Pollen morphological characters were fully appreciated by authors such as Lindley, von Mohl & Fritzsche as early as 1830-1840. But generally, these characters are overlooked by taxonomist due to lack of suf icient data. Recently, however, considerable attention is being paid to pollen morphology, and the science of palynology (study of spores & pollen grains) has entered into current taxonomic thinking.
irst comprehensive book on palynology was written by Wodehouse (1935) & subsequently Erdtman contributed a treatise on pollen analysis (1943). The irst manual, Pollen Morphology and plant Taxonomy, appeared in 1952 which deals with angiosperm [1]. This book provided a summary of pollen morphological characteristics of all angiosperm families. As result of the work of these early authors there has recently been a great increase in the comparative analytical studies of pollen morphology and wall structure. Results of considerable systematic and phylogenetics value have been obtained and the conclusions drawn from such studies are generally in agreement with those drawn from other ields of study.

Characters of pollen grains & their role as plant identifi cation in taxonomy and pharmaceutical value as detection of adulterations in crud drugs
The main characters of taxonomic value in pollen grains are Most angiospermous pollen grain fall into only two general types, Uniaperturate & triaperturate, from which some less common types have been derived. Uniaperturate pollen grain have a single germinal furrow or pore situated at the proximal or distal position. The proximal form of apertures is always found in pteridophyta but in pteridosperms, the position is reported to be either proximal or distal (Potonie, 1967). The proximal aperture is sometimes found in primitive angiosperms (Nair, 1968 The taxonomic & evolutionary importance of pollen morphology may be at speci ic, generic or higher levels. In many cases the types of pollen of a taxon is characteristic & constant. Such a taxon is termed stenopalynousor unipalynous [2], & may be exclusive of that group, e.g. thick walled grains of the Gyrostemonaceae of Australia. In other cases, the types of pollen may vary considerably in size, aperture, strati ication of exine, etc. Such taxa are termed euirypalynous or multipalynous. Stenopalynous taxa are generally considered to be very natural. Asclepiadaceae, Cruciferae, Gramineae, Labiatae, etc. are some of the stenopalynous families. Eurypalynous taxa, on the other hand, are taken to be heterogeneous, at least in certain instances. Palynological data are particularly useful in the delimitation of eurypalynous taxa. Families such as Rubiaceae, Acanthaceae, and Verbenaceae are highly eurypalynous and a revised classi ication of some of these has been proposed by some taxonomists (Bremekamp, 1944(Bremekamp, , 1950

Methodology
The pollens were prepared for light microscopy by standard method. Pollen materials were separated from their anthers with the help of forceps and needle under a dissecting microscope. The pollen grains ready for microscopic observations by acetolysis method and were placed on slide. The measurements were based on 15-20 readings for every specimen. Pollen characteristics of pollen grains as shape, color, exine ornamentations, type of apertures.
Regular ield tours were arranged to collect the plants in lowering condition for preparing pollen grain slides. Collected plants from the ields were identi ied with the help of loras [3][4][5].
In the present work 42 angiosperm plants species are arranged systematically with their botanical names followed by family and characteristic of pollen grains viz. shape, color, exine ornamentation and type of aperture [9,10] (Table 1).

Discussion
In present study, pollen grain morphology of 42 plant species studied. The microscopic observations under light microscope revealed that pollen grains of plants are psilate, rugate to spinulose, verrucate, reticulate, bacculate (bottle shape), spiny, psilate (with smooth surfaces).
The evaluation of crude drug which eventually enters the commercial market is obviously of considerable importance. The pharmacognostic studies consist of the collection of various parts of these plants, their identi ication, standardization, and authentication through various taxonomic markers and macro-and microscopic characters. Companies involved in the crude drug sale generally avoid special recommendations on the use of a product. There are no applicable standards of quality for crude drugs, and they are not usually standardized with respect to the concentration of active components. So, it is necessary to target such crude drugs to various standards of quality, purity, and safety, if acceptable consumer usage is to be achieved. To authenticate crud drugs it is very necessary to go through microscopic scienti ic studies e.g palynological data.

Conclusion
Present work will be very helpful for writing pollen lora of local, regional, state & even pollen lora of the country. This work is important for researchers, palynologist & graduate & post graduate students for identi ication of plant on the basis of simply pollen grains. This palynological work is most important for forensic science to detect the crime on the basis of pollen grains & spores. Pollen morphology has been regarded as a taxonomic character of high signi icance & used the character along with basic chromosome numbers as a basis for circumscribing groups of genera. This palynological work is also very important for evolutionary studies and to detect adulterations in crude drugs of plant origin. https://doi.org/10.29328/journal.jpsp.1001046