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Submitted: 20 February 2020 | Approved: 01 April 2020 | Published: 02 April 2020

How to cite this article: Ragho KS. Role of pollen morphology in taxonomy and detection of adulterations in crud drugs. J Plant Sci Phytopathol. 2020; 4: 024-027.

DOI: 10.29328/journal.jpsp.1001046

Copyright: © 2020 Ragho KS. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords: Pollen morphology; Taxonomy; Plants; Drug adulterations; India

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Role of pollen morphology in taxonomy and detection of adulterations in crud drugs

Kshirsagar Sanjay Ragho*

Post Graduate, Department of Botany, Shri Shivaji Vidya Prasarak Sanstha’s Late Karmveer, Dr. P.R. Ghogrey Science College, Maharashtra, India

*Address for Correspondence: Kshirsagar Sanjay Ragho, Post Graduate Department of Botany, Shri Shivaji Vidya Prasarak Sanstha’s Late Karmveer, Dr. P.R. Ghogrey Science College, Maharashtra, India, Email: srktaxon1969@rediffmail.com

Present paper communicates 42 species of angiosperms depicting characteristics of pollen grains as shape, color, exine ornamentations, and type of apertures. Pollen morphological characters are very important in plant identifications in field. Pollen surface features plays significant role in taxonomy and detection of crud drugs. Firsthand information is gathered from field and provided in this research article.

The use of herbal medicine for the treatment of diseases and infections is a safe and traditional therapy.

Pollen morphological characters are used to identify adulterations in crud drugs or dry herbs of some medicinal plants as pollen grains are very minute and are adhered in any part of the crud drugs.

In developing countries, medicinal plants are attaining greater importance in the primary health care of individuals and communities. Medicinal plants form a large group of economically important plants that provide the basic raw materials for indigenous pharmaceuticals

Pollen characters are useful in solving complicated problems of interrelationships between various taxa and assessment of their status in the classification, particularly with reference to the families, subfamilies, tribes, genera, species, and subspecies. Pollen morphology is conducted as an aid to the morphological study and a significant tool for modern taxonomist for the delimitation of species. Mature pollen grain size, exine sculpturing, and number of pores are the most distinctive features. Palynological data has been useful at generic and specific level. This analysis also helps in qualitative analysis of drug powder and the correct identification of drug. It plays an important role in our daily life as well. Aerobiology has received much attention due to its wide application in allergology, forestry, agriculture, horticulture, archaeology, and plant geography.

Pollen morphological characters were fully appreciated by authors such as Lindley, von Mohl & Fritzsche as early as 1830-1840. But generally, these characters are overlooked by taxonomist due to lack of sufficient data. Recently, however, considerable attention is being paid to pollen morphology, and the science of palynology (study of spores & pollen grains) has entered into current taxonomic thinking.

First comprehensive book on palynology was written by Wodehouse (1935) & subsequently Erdtman contributed a treatise on pollen analysis (1943). The first manual, Pollen Morphology and plant Taxonomy, appeared in 1952 which deals with angiosperm [1]. This book provided a summary of pollen morphological characteristics of all angiosperm families. As result of the work of these early authors there has recently been a great increase in the comparative analytical studies of pollen morphology and wall structure. Results of considerable systematic and phylogenetics value have been obtained and the conclusions drawn from such studies are generally in agreement with those drawn from other fields of study.

Characters of pollen grains & their role as plant identification in taxonomy and pharmaceutical value as detection of adulterations in crud drugs

The main characters of taxonomic value in pollen grains are number & position of furrows, number, position of complexity of apertures & form of sculpturing of the exine. Variations is also shown in size & general types.

Most angiospermous pollen grain fall into only two general types, Uniaperturate & triaperturate, from which some less common types have been derived. Uniaperturate pollen grain have a single germinal furrow or pore situated at the proximal or distal position. The proximal form of apertures is always found in pteridophyta but in pteridosperms, the position is reported to be either proximal or distal (Potonie, 1967). The proximal aperture is sometimes found in primitive angiosperms (Nair, 1968), but the distal position is predominant in flowering plants. Uniaperturate pollen grains are characteristics of the monocotyledon & some members of Magnoliidae.

Triaperturate pollen grains have three germinal furrows, either radiating like the lines of a trilete mark from a common point or forming a triangle at the distal position. Various other numbers of furrows & other types of apertures are situated along the equatorial line (zonal position) or distributed over the entire surface (global distribution). The triaperturate or triaperturate-derived pollen grains occur in the bulk of dicotyledons & are unknown elsewhere. Thus, along with the zonal & global position of apertures, evolved many aperturate condition also.

The taxonomic & evolutionary importance of pollen morphology may be at specific, generic or higher levels. In many cases the types of pollen of a taxon is characteristic & constant. Such a taxon is termed stenopalynousor unipalynous [2], & may be exclusive of that group, e.g. thick walled grains of the Gyrostemonaceae of Australia. In other cases, the types of pollen may vary considerably in size, aperture, stratification of exine, etc. Such taxa are termed euirypalynous or multipalynous. Stenopalynous taxa are generally considered to be very natural. Asclepiadaceae, Cruciferae, Gramineae, Labiatae, etc. are some of the stenopalynous families. Eurypalynous taxa, on the other hand, are taken to be heterogeneous, at least in certain instances. Palynological data are particularly useful in the delimitation of eurypalynous taxa. Families such as Rubiaceae, Acanthaceae, and Verbenaceae are highly eurypalynous and a revised classification of some of these has been proposed by some taxonomists (Bremekamp, 1944, 1950).

The pollens were prepared for light microscopy by standard method. Pollen materials were separated from their anthers with the help of forceps and needle under a dissecting microscope. The pollen grains ready for microscopic observations by acetolysis method and were placed on slide. The measurements were based on 15-20 readings for every specimen. Pollen characteristics of pollen grains as shape, color, exine ornamentations, type of apertures.

Regular field tours were arranged to collect the plants in flowering condition for preparing pollen grain slides. Collected plants from the fields were identified with the help of floras [3-5].

Pollen grains mounted & observed under microscope, light microscope, research trinocular & binocular microscopes. Pollen grains were identified with the help of some published literature [1,2,6-9].

In the present work 42 angiosperm plants species are arranged systematically with their botanical names followed by family and characteristic of pollen grains viz. shape, color, exine ornamentation and type of aperture [9,10] (Table 1).

Table 1: Systematic Enumeration of the plants studied.
Sr.
No.
Botanical Names Family Characteristics of Pollen Grains
Shape Color Exine ornamentation Types of apertures
1 Quisqualis indica L. Combretaceae Elliptical Light brown Psilate Colpate
2. Pedilanthus tithymaloides (L.) Poit. Euphorbiaceae Rounded Brown yellow Psilate(smooth) Porate
3. Stachytarpheta jamaicensis (L.)Vahl Verbenaceae Elliptical, rod, rounded, circularin outline White Psilate Porate, colpate
4. Spathodea campanulata Beauv. Bignoniaceae Circular, irregular in outline Light brown Psilate Porate, (trizonoporate, Colporate)
5. Crossandra
undulifolia Salib.
Acanthaceae Rod shaped Light red & yellow Spinulose Bicolpate
6. Ixora parviflora Vahl. Rubiaceae Rounded, circular Light yellow Psilate (smooth) Colporate
7. Hyptis suaveolens (L.) Poit. Lamiaceae Rounded, circular in outline Brownish yellow Psilate Porate
8. Moringa concanensis Nimmo ex Dalz. & Gibs. Moringaceae Elliptic, oval Faint cream Psilate Colpate (monocolpate)
9. Cleome viscosa L. Cleomaceae Elliptic Light yellow Psilate Colpate(bicolpate)
10. Rhynchosia minima (L.) DC. Papilionaceae Slightly triangular in shape, circular Brown, greenish Psilate Porate (Trizonoporate)
11. Lantana camara L. Verbenaceae Rounded Yellow Psilate Porate
12. Azadiracta indica A. Juss. Meliaceae Rounded,elliptical Hyaline Psilate Colpate (bicolpate)
13. Thevetia peruviana
(Pers.) Shetty & Singh
Apocynaceae Triangular, semi-circular, trichotomous Light yellow Psilate Porate(trizonoporate)
14. Euphorbia indica Lam. Euphorbiaceae Elliptic Light yellow Psilate Colpate
15. Pyrostegia venusta Miers. Bignoniaceae Elliptic, oval Light yellow Psilate Colpate (bicolpate)
16. Euphorbia millii Des. moul. Ephorbiaceae Elliptic, circular, oval Light brown Psilate (thick eticulate) Colpate (bicolpate)
17. Tecoma stans (L.) Juss. Ex Kunth Bignoniaceae Rounded, elliptical,circular Yellow brown Psilate Colpate (tricolpate)
18. Justicia gendarussa Burm. f. Acanthaceae Elliptic, oval,circular in outline Light yellow Psilate Porate, colpate (triporate)
19. Ipomoea obscura (L.) Ker.-Gawl. Convolulaceae rounded / circular in outline Light brown Spiny Porate (Pantoporate)
20. Sonchus oleraceus L. Compositae Quadranguler trianguler Light yellow Spinulate Porate(Pantoporate, trizonoporate)
21. Clitoria ternatea L. Papilionaceae Quadrangular,triangular Light gray Psilate Porate (broad colpi)
22. Datura innoxia Mill. Solanaceae More / less rounded Light gray Psilate (thick) Porate (Trizonoporate)
24. Jatropha pandurifolia Andr. Euphorbiaceae Circular /rounded Light yellow Rugulate, reticulate (thick) Porate
25. Alternanthera
sessilis (L.).R.Br. ex DC.
Amaranthaceae More/ less circular in outline Light yellow Psilate Porate(Trichotomous)
26. Launaea
Procumbens
(Roxb.) Ramayya & Rajgopal
Compositae Triangular, Semi circular Light yellow Verucate (reticulate) Porate (Trichotomoproate)
27. Plumbago
zeylanica L.
Plumbaginaceae Rounded Light yellow Psilate Porate (Trizonoporate)
28. Oscimum sanctum L. Labiatae Rounded Light yellow Short Spiny Porate
29. Ocimum basilicum L. Labiatae Rounded Cream white blackish /Hyaline Reticulate Porate(pantoporate)
30. Portulaca quadrifida L. Portulaceae Rounded,circular (double layer) Yellowish Spiny Porate(pantoporate)
31. Sida cordifolia L. Malvaceae Rounded Brown Spiny Porate (Pantoporate)
32. Rhoeo discolor (L.) Hance Commelinaceae Rod shape White Psilate Colpate
33. Hibiscus rosa-sinensis L. Malvaceae Rounded or circular in outline Light brown Bacculate(bottle shape) Porate (pantoporate)
34 Sida acuta Burm.f. Malvaceae More/less rounded Light brown Rugate to Spinulose Porate (pantoporate)
35 Vinca rosea L. Apocynaceae Rounded / circular Light yellow, pinkish at border   Porate
36 Bougainvillea spectabilis Willd. Nyctaginaceae Rounded White brown   Spinulose
37 Asystasia dalzelliana Santapau Acanthaceae Rod shape / elliptical Faint white Psilate (smooth) Porate (Dizonoporate)
39 Lindenbergia macrostachya (Benth.) Benth. Scrophulariaceae Elliptical White-hyaline Psilate Colpate
40 Vernonia cinerea (L.) Less. Compositae Circular/rounded in outline Faint brown Verrucate, reticulate Porate(Trizonoporate)
41. Tridax procumbens L. Compositae Quadrate/more or less circular in utline Light black Spiny Porate (Pantoporate)
42. Euphorbia clarkeana Hook. f. Euphorbiaceae Rounded Light brown Psilate Porate (aperture on distal position)

In present study, pollen grain morphology of 42 plant species studied. The microscopic observations under light microscope revealed that pollen grains of plants are psilate, rugate to spinulose, verrucate, reticulate, bacculate (bottle shape), spiny, psilate (with smooth surfaces).

The evaluation of crude drug which eventually enters the commercial market is obviously of considerable importance. The pharmacognostic studies consist of the collection of various parts of these plants, their identification, standardization, and authentication through various taxonomic markers and macro- and microscopic characters. Companies involved in the crude drug sale generally avoid special recommendations on the use of a product. There are no applicable standards of quality for crude drugs, and they are not usually standardized with respect to the concentration of active components. So, it is necessary to target such crude drugs to various standards of quality, purity, and safety, if acceptable consumer usage is to be achieved. To authenticate crud drugs it is very necessary to go through microscopic scientific studies e.g palynological data.

Present work will be very helpful for writing pollen flora of local, regional, state & even pollen flora of the country. This work is important for researchers, palynologist & graduate & post graduate students for identification of plant on the basis of simply pollen grains. This palynological work is most important for forensic science to detect the crime on the basis of pollen grains & spores. Pollen morphology has been regarded as a taxonomic character of high significance & used the character along with basic chromosome numbers as a basis for circumscribing groups of genera. This palynological work is also very important for evolutionary studies and to detect adulterations in crude drugs of plant origin.

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