Volume 1 Issue 1
2016-12-30 Research Article
Concentration Polarization of Ox-LDL and Its Effect on Cell Proliferation and Apoptosis in Human Endothelial Cells
Background: Flow-dependent concentration polarization of native LDL is important in the localization of atherogenesis. However, ox-LDL plays a more important role than n-LDL in atherogenesis by inducing cell proliferation and apoptosis. We hypothesized that concentration polarization of ox-LDL may adversely affect vascular beds due to its toxicity to endothelial cell (EC) lining.
Methods: Using a parallel-plate flow chamber technique, we studied water filtration rate and wall concentration of ox-LDLs EC monolayers cultured on permeable or non-permeable membranes. ECs cultured on permeable and non-permeable membranes were examined in terms of cell viability, ox-LDL uptake, LOX-1 expression and cell apoptosis (Cytochrome c and Bcl-2 expression). We observed that the wall concentration of ox-LDL was about 16% higher in the permeable group than in the permeable group (P<0.05). Cell proliferation (MTT assay) increased in response to low concentration of ox-LDL (1-5 μg/ml), and fell drastically in response to higher concentration; all these changes were more pronounced in the permeable group than in the non-permeable group. The uptake of ox-LDL and LOX-1 expression by ECs were also significantly higher in the permeable group than in the non-permeable group of cultured cells.
Conclusions: These observations suggest that concentration polarization of ox-LDL occurs in an artery that is permeable to water, and ox-LDL concentration polarization can enhance ox-LDL accumulation into the arterial wall and accelerate EC proliferation at low concentrations and apoptosis at high concentrations, possibly via LOX-1 expression.
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001003 Cite this Article
2016-12-30 Letter to the Editor
Mid-Ventricular Ballooning in Atherosclerotic and Non-Atherosclerotic Abnormalities of the Left Anterior Descending Coronary Artery
Letter to Editor
Myocardial coronary bridging , encasement , and mid-LAD rigid straightening [3-5] appears to be epiphenomenon of takotsubo syndrome , rather than causing the disease. Typical takotsubo syndrome usually lasts only a few weeks, but chronic takotsubo cardiomyopathy is reported .
In a series of meanwhile 10 cases rigid straightening of the mid-portion of the left anterior descending coronary artery without lumen reduction mid-ventricular or basal ballooning was reported, or both basal and mid-ventricular ballooning in one case . In all these patients wrap-around (recurrent segment) phenomenon of the left anterior descending coronary artery was not present. The abnormalities of the left anterior descending coronary artery are due to myocardial bridging without lumen reduction of the LAD, only seen in computed tomography . When stress or in some cases happiness appears myocardial ballooning can appear, lasts 2-4 weeks and disappear with a recurrence rate of nearly 10% despite beta blocking agents .
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001002 Cite this Article
2016-12-07 Research Article
Left Atrial Remodeling is Associated with Left Ventricular Remodeling in Patients with Reperfused Acute Myocardial Infarction
Background: Left atrial volume (LAV) has been established as a sensitive marker of left ventricular (LV) diastolic function and as an independent predictor of mortality in patients with acute myocardial infarction (AMI). LA remodeling and its determinants in the setting of AMI have not been much studied.
Methods: We studied 53 patients with anterior AMI and a relatively preserved LV systolic function, who underwent complete reperfusion and received guidelines guided antiremodeling drug management. LA and LV remodeling were assessed using 2D echocardiography at baseline and 6 months. LAV indexed for BSA (LAVi) was used as the index of LA size and further LA remodeling.
Results: LAVi increased signifi cantly at 6 months compared to baseline [28.1 (23.0-34.5) vs 24.4 (19.5- 31.6) ml/m2, p=0.002] following LV end diastolic-volume index change [56.8 (47.6-63.9) vs 49.5 (42.0-58.4) ml/m2, p=0.0003]. Other standard LV diastolic function indices did not show any signifi cant change. Univariateanalysis showed a strong positive correlation of LAVi change with BNP levels at discharge, LV mass index and LV volumes indices change, throughout the follow up period. Multivariate regression analysis revealed that BNP plasma levels was the most important independent predictor of LA remodeling (b-coef.=0.630, p=0.001).
Conclusions: Despite current antiremodeling strategies in patients with AMI, LA remodeling is frequently asssociated with LV remodeling. Additionally LAVi change in the mid-term reflects better than standard echocardiographic indices LV diastolic filling impairment.
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001001 Cite this Article
2021-07-09 Research Article
Open heart surgery in Nigerian children the need for international and regional collaboration: The Bayelsa and Enugu experience
Ujunwa FA, Ujuanbi AS, Chinawa JM*, Allagoa DO and Onwubere B
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001117 Citation
2021-05-05 Case Report
Lamanna B, Vinciguerra M, Crupano F, Cicinelli R, Cicinelli E and Vimercati A
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001116 Citation
2021-04-23 Review Article
Seriki Samuel Adinoyi*
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001115 Citation
2021-04-16 Case Report
Anshuman Das, Maria Isabel Camara Planek and Steve Attanasio*
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001114 Citation
2021-03-24 Research Article
Abdominal obesity in predicting myocardial infarction risk. Waist-to-Hip Ratio: The metric that confused cardiology worldwide for a long time
Angel Martin Castellanos*
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001113 Citation
2021-03-19 Review Article
Nelson I Oguanobi*
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001112 Citation
2021-03-02 Research Article
Gerry A Smith*
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001111 Citation
2021-02-12 Case Report
Florina-Adriana Ghitun, Stefan Ailoaei, Dan Ursu, Raluca Chistol, Grigore Tinica, Cristian Statescu and Mihaela Grecu
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001110 Citation
2021-01-15 Review Article
Gerry A Smith*
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001109 Citation
2021-01-05 Research Article
Mirzaie Masoud*, Zaur Guliyev, Michael Schultz, Peter Schwartz, Johann Philipp Addicks and Sheila Fatehpur
Abstract Read Full Article HTML DOI: 10.29328/journal.jccm.1001108 Citation