Comparative Osteometric study of some selected bones of local domestic turkey and guinea fowl

Avian also known as birds are a group of endothermic vertebrates whose characteristics include feathers, toothless beaked jaws, laying of hard-shelled eggs, four chambered hearts and a strong but lightweight skeleton. Avian live all around the world and vary in size with the smallest being the bee hummingbird with a height of 2 cm and the largest being the ostrich with a height of 2.75 m. There are approximately ten thousand species (10,000) of birds around the whole world [1]. Chickens and turkeys are the most commonly reared avian because of their high economical and health importance to humans [1].


Introduction
Avian also known as birds are a group of endothermic vertebrates whose characteristics include feathers, toothless beaked jaws, laying of hard-shelled eggs, four chambered hearts and a strong but lightweight skeleton. Avian live all around the world and vary in size with the smallest being the bee hummingbird with a height of 2 cm and the largest being the ostrich with a height of 2.75 m. There are approximately ten thousand species (10,000) of birds around the whole world [1]. Chickens and turkeys are the most commonly reared avian because of their high economical and health importance to humans [1].
Osteology is the study of the structures and function of the skeleton and bony structures. It is the scienti ic study of bones it is a sub discipline of anatomy. Bones make up a skeleton; they are designed to provide adequate strength with minimal mass or weight. Based on development, we have Endochondral and Intramembranous bones. Based on location, are classi ied based on Axial and Appendicular skeleton, while based on shape, we have long bones short bones, lat bones, irregular bones and sesamoid bones.
The Skeleton is de ined as the frame work that supports, protects and forecasts the body structure of an organism [2]. The skeleton can be classi ied into two based on their location as internal and external skeletons. Skeleton come in

Abstract
The research was conducted in the Gross section of Veterinary Anatomy laboratory with the aim of preparation and comparing some skeleton bones of local domestic turkey and guinea fowl. Samples were purchased, sacrifi ce, feather and excess fl esh were removed and boiled using water to produce the bones. The duration of process was recorded. Comparative biometry study was conducted on some selected bones (scapular, coracoid, furcular and tibiotarsus) and the bones were mounted using wooden stand, copper wire, and adhesive gum with the aim of enhancing avian teaching. Based on the processes of the research. It was recommended to use plastic materials in production of skeletal models to avoid deterioration of bones for proper teaching in veterinary anatomy. a number of forms, each suited for a particular set of lifestyles and environment. Skeletons can be rigid, semi-rigid or soft. vertebrates have internal skeleton called bony skeleton, which consist mainly of calci ied bone tissues The internal skeletons are hard mineralized structures that are located within the muscles of the organisms. The internal skeletons provide protection, support and enable movement of the muscle. The external skeletons are hard encasements on the surface of organisms, insects and animals. Examples of external skeleton include shells of crabs and insects. This external skeleton provides defense against predators, allows for movement and supports the body [1].
The skeleton is composed of bones, cartilages and ligaments. Skeletons of animals and birds are the basic need of the veterinary study, especially for the veterinary gross anatomy [3]. These skeletons are necessary for research ranging from phylogenic investigations to age and growth analyses to functional morphology [4] and essential tools for the study of systematic, biomechanics, evolutionary morphology & adaptation, paleontology and identi ication of animal remains from archeological sites. It is the backbone of the study of many pathological conditions. It is the backbone of the study of many pathological conditions [4].
Turkey is an avian specie (Meleagris gallopavo) which is a large gallinaceous bird of the family Meleagridae, which is a native of North America. There is only one turkey breed but there are many turkey species. The popular varieties that have attained commercial importance in different parts of the world are the Broad Breasted, Large White and the Broad Breasted Bronze. Other breeds are the White Holland, the Beltsville Small White, the Black, the Bourbon Red and the Narrangansell [5]. Male turkeys have a distinctive leshy wattle or protuberance that hangs from the top of the beak called a (snood). They are among the largest birds in their ranges. As in many Galliformes, the males are larger and much more colorful than females. Turkeys are classed in the family phasianidae in the taxonomic order of meleagris ocellata. The meleagris is the only extent genus in the subfamily meleagrididae, but now subsumed within the family phasianidae. The two main species are distributed thus: meleagris gallopavo also known as domestic turkey or wild turkey are found in forests of North America, Throughout the Midwest and in southern Canada meleagris ocellata also known as ocellated turkey are found in the forests of the Yucatan peninsula [6]. Turkeys are reared using a domesticated system in Europe, a semi domesticated system and free range system in Africa [7]. Turkeys have an estimated population of over seven million turkey's worldwide [6].
Guinea fowls are the largest living bird reaching over 200 kg body weight and 2.7cm in height. It is classi ied under Ratites, which are large ground dwelling, lightless birds such as rhea, emu, cassowary, and kiwi. Guinea fowls also known as original fowl are birds of the family numididae in the order Galliformes. They are endemic to Africa and rank among the oldest of the gallinaceous birds. Phylogenetically, they branch off from the core Galliformes after the cracidae and before the odontophoridae, which is an Eocene fossil lineage, telecrex [8]. The insect eating, ground nesting birds of this family resemble partridges, but with featherless heads, though both members of the genus guttera have a distinctive black crest, and the vulturine Guinea fowl has a downy brown patch on the nape. Most species of Guinea fowl have a dark grey or blackish plumage, with dense white spot, but both members of the genus angulates lack the spots.
Skeleton of animals and birds are the basic need of the veterinary study, especially for the veterinary gross anatomy [3]. These skeletons are necessary for research ranging from phylogenic investigations to age and growth analyses to functional morphology [4] and essential tools for the study of systematic, biomechanics, evolutionary morphology and adaptation, paleontology and identi ication of animal remains from archeological sites. It is the backbone of the study of many pathological conditions. It is the study of many pathological conditions [4]. This work is done to make available the morphometrical studies of the bones of an adult turkey and guinea fowl for analysis and comparative studies in the study of the skeletal anatomy of bones. The aim of the research is to compare Morphological and biometric parameters of some selected bones of clinical importance in an adult local domestic turkey and guinea fowl..

Study area
The city of Sokoto is located in the extreme North West of Nigeria near to the con luence of river Sokoto and River Rima. Sokoto has a land size of 25,973 km 2 with population of 3,702,676 people. Sokoto state shares a boarder with Niger Republic, Zamfara state and Kebbi State.
The northwestern state was created in 1967, seven years after Nigeria got her independence from the Britain. It was later split into Sokoto and Niger and Sokoto was then split into Kebbi and Zamfara in 1991 and 1996 respectively. The state has an annual average temperature of 28.3, making it one of the hottest cities in the world, however the maximum daytime temperatures are generally under 40 °C most of the year although it could go above. Sokoto currently is the second largest producer of livestock in Nigeria. With an estimate of 1.18 million cattle, 2.90 million goats, 1.90 million sheep, 2.0 million chickens and turkeys, 45,000 camels, 34,532 horses and 51,388 donkeys.

Sample collection and processing
Ten (10) fully grown and matured turkey of age 2-3 years and ten (10) fully-grown matured guinea fowl of age 2-3 years were selected based on their history, physical appearances and lack of any skeletal abnormalities. These breeds were all obtain from the local houses in Sokoto town. It was ensured that there were no congenital deformities and scars present in the samples. The avian were later processed for biometric comparative analysis in the gross Anatomy Laboratory of the Faculty of Veterinary Medicine at Usmanu Danfodiyo University Sokoto, Sokoto State.
The birds were then sacri iced by the severing of the jugular veins; great care was taken so as not to damage the bones of the neck. They were then de-feathered by emancipation in hot water and the feathers were then plucked out. The internal organs of the birds were gently removed after de-feathering had being done. The birds were then de-leshed using a sharp scalpel blade, carefully removing the feathers from the thighs, the pectoral muscles and muscles from the wings. The remaining adhering muscles were removed by hot water and maceration technique. The turkey and guinea fowl were then boiled separately and a little of potash was added to help in the loosening of the lesh. The boiling lasted for45 minutes. After boiling, the lesh of the birds was removed gently using scalpels blades, forceps, knives and hands. The bones were then soaked in water for four days. Great care was taken so as to avoid damage to the bones from boiling.
The bones were then rinsed and washed with detergent and then dried in the open for 3 days. After drying the bones were then treated with bleaching reagents (hydrogen peroxide) so as to prevent them from decaying and to prevent insects from burrowing into them, the bones were soaked in water and hydrogen peroxide solution for four days and then kept out to dry for two days.
A biometric study on a comparative study of the turkey and guinea fowl was then carried out by taking certain measurements of selected number of bones of the turkey and guinea fowl using meter rule, digital Vernier caliper, strong white thread, digital weighing balance. The results of this measurements are shown in tables 1-4. After taking the measurements the bones were then assembled and the skeleton was mounted.

Materials used
After successful separation and processing of bones, the bones were measured and mounted afterwards using the following materials. The digital caliper is a precision instrument that can be used to measure internal and external distances extremely accurately, it is used by sliding the jaw along the main scale, the rectangular plates align and misaligns and the capacitance (the amount of electrical charge) between the plates changes. This sends a signal to a chip within the caliper, which generates the readings shown on the LCD display. Calipers usually give a precision to 0.01 mm (10 micrometers), or one thousandth of an inch. It has a resolution of 0.1mm and accuracy of 0. 2 mm. Digital weighing balance also known as beam balance was used to take the weights of the selected bones measured, it is a simple device. The bones were placed on the plate of the weighing balance and the readings were read from the LED display monitor.
Thread and meter rule were used simultaneously. The thread was used in measuring curved and rough areas of the bones and then placed on the meter rule to ascertain the measurement. The meter rule was used in straight bones and bones with few rough and less curved surfaces majorly to determine the length of such bones.

Biometric
This is the application of statistical data to biological data, it is the measurement and statistical analysis of unique physical and behavioral characteristics. In this study, it involves the statistical analysis between selected bones of an adult turkey and an adult guinea fowl, the use of standard error of mean also known as standard deviation and a software known as statistical analysis system to determine the difference and the accuracy of the measurements taken.

Bones used
The bones used for the comparative study in this research work are the

Standard error of mean:
The standard error is a statistical term that measures the accuracy with which a sample distribution represents a population by using standard deviation. In statistics, a sample mean deviates from the actual mean of a population this deviation is the standard error of the mean SAS is a command-driven software package used for statistical analysis and data visualization.
Photograph: Photograph of the bones and mounted skeleton were taken using Samsung digital camera 16.1mp.

Gross observation
The results from the study of the morphometry and biometric of selected bones of both adult turkey and guinea fowl are reported below.
After gross comparison of the selected bones from the turkey and guinea fowl (tibiotarsus, coracoid, scapula, and the furcular) the following observations were made and differences noticed concerning the furcular; The furcular of turkeys was found to be clearly triangular in shape and both clavicles are lattened with an inverted groove at the medial side. The inter clavicle is pointed ventrally.
The furcular of guinea fowls is slightly triangular in shape but both clavicles are curved in a convex manner cranially. The edges at the proximal part tapers and the inter-clavicle are bilaterally lattened (Plates 1-5).

Discussion
The study revealed that most studies carried out that involve preparation and mounting of skeleton of avian species used water and heat maceration [9] and use of chemicals such as sodium perforate. The preparation involves skinning, removal of the skin completely, removal of viscera and other soft organs before removal of muscles close to their attachment and insertion into the bones and cartilages, disarticulation and cleaning using water and a pair of forceps. Bleaching and curing to preserve the bones using either chlorine solution, hydrogen peroxide or lime water [10]. The most fully documented historical account of skeleton production is that of the Smithsonian institute, with his formula of 72-hour hot water soaking, brushing, and the use of benzene, which has proven to be highly toxic.
The assemblage of avian skeleton involves consulting a diagram of the said avian anatomy as done in this study to ensure proper assemblage of the skeleton. Snyder, Burdi, and Gaul introduced a method of skeletal preparation that involved a quick acting formula they named anti-forming, prepared by combining sodium carbonate and bleaching powder. The authors note that other effective means are time consuming. They state that the ive-step method of maceration, cleaning, degreasing, bleaching, and ixing as well as the use of domestic beetles are, in effect, not necessary [2]. Synder notes that hydrogen peroxide and potassium hydroxide are especially useful for bones that may crack or degrade from repeated boiling and scraping, such as skull and scapula. Another method discussed that may help to avoid decalci ication is macerating uncovered in clear water for a few days to weeks, followed by immersion in a detergent solution and simmered [11].
Detergent maceration makes use of the enzymes present in the cleaning agent, and an increased speed of maceration and       removal of bad smell have been observed. However, key here is that the exact composition of commercial detergents is often proprietary and not directly available. Besides various kinds of enzymes, the detergents also contain ten sides, builders (inorganic complexing agents), additives, bleaching agents and corrosion inhibitors. The aggressive mixture in detergents may cause damage to specimens and decalci ication, softening and transparency of detergent-macerated bones.
Yin, et al. warned of the adverse effects of enzymatic maceration on hardness of bones, and a year later [12] applied various concentrations of lipase and protease, with successful results reported. Having said that, the materials and methods of four works reviewed associate in a similar mindset as each experiment builds on those whose research came before. Each scientist modi ies previous works. Reducing bone modi ications as well as increasing potential genetic material have become primary goals. While summary reviews are enumerated, the one point that all agree on is the negative effect of bleaching with sodium hypochlorite. All caution against its use, warning that resultant bone will be lanky and at times crumble quickly to dust. They instead suggest hydrogen peroxide or potassium hydroxide, which was used during this study.

Conclusion
The study revealed that the measured parameters have been individually evaluated for both left and right sides of the turkey and guinea fowl using two replications per treatment. The data were analyzed using Duncan system grouping and mixed procedure of statistical analysis system. The signi icant differences between means were detected using Duncan's multiple range tests. The standard error of mean is set at p < 0.05. Mounting of avian skeleton is possible but requires the use of an aged (old) bird for the betterment of necessary anatomical features.
Comparative anatomical features such as foramina, crest, depressions, pneumatization, and ridges might be seen in some species and not seen in others.
The most recommended lesh removal method is the maceration technique and the use of hydrogen peroxide and potassium hydroxide instead of sodium hypochlorite which is known to weaken the bone and make it lanky.

Recommendation
The following recommendations were made for future research purposes. The need to use plastic models in the production of skeletal elements in enhancing veterinary anatomical teachings. Comparative analysis should be done on different lesh removal techniques in order to know which is more ef icient and the use of different chemicals to ensure the specimen remain intact. Comparative analysis between breeds of local turkey, local chicken and guinea fowl should be done to see if there are differences in the anatomical features. Comparative analysis should be done on both sexes of the turkey breed of a given age. The use of other measuring and biometrical equipment's should be used when carrying out further research.