Research Article

Effects of KCl (rpm/Heat) on Bacterial Protease Production in E. coli, P. aeruginosa and E. faecalis

Canan Cennet Karaderi and Huseyin Kahraman*

Published: 09/11/2018 | Volume 2 - Issue 1 | Pages: 012-017

Abstract

Background: Proteases are a group of enzymes that catalyze the cleavage of peptide bonds in proteins found in nature. Microbial protease constitutes one of the most important for industrial aplications. Proteases play a crucial role in numerous pathologic processes as well. KCl is an unnatural salt. The purpose of this study was to examine the effect of this salt on protease production under different agitation and heat conditions.

Methods: The effects of KCl (rpm/heat) on the production of a protease, of E. coli, P. aeruginosa and E. faecalis strain, were investigated. The decrease in protease production at 37 °C was also observed in this work that proved that heat plays a major role in enzyme production.

Results: The presence of KCl also caused a decrease in protease production in three bacterial species. The use of KCl appears to be a viable alternative when it is necessary to reduce protease activity outside of industrial applications (such as health care). This unique property makes it attractive and useful to be used in health industries. In the future we think that it will contribute to clarification of the matter in this way.

Read Full Article HTML DOI: 10.29328/journal.abb.1001011 Cite this Article

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