Research Article

Effects of KCl (rpm/Heat) on Bacterial Protease Production in E. coli, P. aeruginosa and E. faecalis

Canan Cennet Karaderi and Huseyin Kahraman*

Published: 09/11/2018 | Volume 2 - Issue 1 | Pages: 012-017


Background: Proteases are a group of enzymes that catalyze the cleavage of peptide bonds in proteins found in nature. Microbial protease constitutes one of the most important for industrial aplications. Proteases play a crucial role in numerous pathologic processes as well. KCl is an unnatural salt. The purpose of this study was to examine the effect of this salt on protease production under different agitation and heat conditions.

Methods: The effects of KCl (rpm/heat) on the production of a protease, of E. coli, P. aeruginosa and E. faecalis strain, were investigated. The decrease in protease production at 37 °C was also observed in this work that proved that heat plays a major role in enzyme production.

Results: The presence of KCl also caused a decrease in protease production in three bacterial species. The use of KCl appears to be a viable alternative when it is necessary to reduce protease activity outside of industrial applications (such as health care). This unique property makes it attractive and useful to be used in health industries. In the future we think that it will contribute to clarification of the matter in this way.

Read Full Article HTML DOI: 10.29328/ Cite this Article


  1. Tekin N, Cihan AC, Takaç ZS, Tüzün CY, Tunç K, et al. Alkaline protease production of Bacillus cohnii APT5. Turk J Biol. 2012; 36: 430 - 440. Ref.:
  2. Kıran ÖE, Çömlekçioğlu U, Dostbil N. Some microbial enzymes and usage fields in industry. KSU J Sci Eng. 2006; 9: 12-19.
  3. Abusham RA, Rahman RNZRA, Salleh AB, Basri M. Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand. Microbial Cell Fact. 2009; 8: 20. Ref.:
  4. Ramalakshmi N, Narendra D, Ramalakshmi M, Roja S, Archana BKN, et al. Isolation and characterization of protease producing bacterial from soil and estimation of protease by spectrophotometer. The Exp. 2012; 1: 1-7. Ref.:
  5. Saleem AJ. Relationship study between the alkaline protease production and the growth phases of Pseudomonas aeruginosa isolated from patients. Adv Microbiol. 2012; 2: 354-357. Ref.:
  6. Ariole CN, Ilega E. Alkaline protease production by Pseudomonas aeruginosa isolated from the gut of Pila ovata. J Glob Biosci. 2013; 2: 126-131. Ref.:
  7. Vermelho AB, Meirelles MNL, Lopes A, Petinate SDG, Chaia AA, et al. Detection of extracellular proteases from microorganisms on agar plates. Mem I Oswaldo Cruz. 1996; 91: 755-760. Ref.:
  8. Maal KB, Emtiazi G, Nahvi I. Production of alkaline protease by Bacillus cereus and Bacillus polymixa in new industrial culture mediums and its immobilization. Afr J Microbiol Res. 2009; 3: 491-497. Ref.:
  9. Alnahdi HS. Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp. J Appl Pharm Sci. 2012; 2: 71-74. Ref.:
  10. Smita GS, Ray P, Mohapatra S. Quantification and optimisation of bacterial isolates for production of alkaline protease. Asi J Exp Biol Sci. 2012; 3: 180-186.
  11. Anbu P. Enhanced production and organic solvent stability of a protease from Brevibacillus laterosporus strain PAP04. Brazil J Med Biol Res. 2016; 49: 1-7. Ref.:
  12. Andrejko M1, Zdybicka-Barabas A, Janczarek M, Cytryńska M. Three Pseudomonas aeruginosa strains with different protease profiles. ACTA Biochim Pol. 2013; 60: 83-90. Ref.:
  13. Haddadi K, Moussaoui F, Hebia I, Laurent F, Roux YL. E. coli proteolytic activity in milk and casein breakdown. Reprod Nutr Dev. 2005; 45: 485-496. Ref.:
  14. Mahanta N, Gupta A, Khare SK. Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Biores Technol. 2008; 99: 1729-1735. Ref.:
  15. Maghsoodi V, Kazemi A, Nahid P, Yaghmaei S, Sabzevari MA. Alkaline protease production by immobilized cells using B. licheniformis. Sci Iran C. 2013; 20: 607-610. Ref.:
  16. Liburdi K, Benucci I, Esti M. Study of two different immobilized acid proteases for wine application. Food Biotechnol. 2010; 24: 282-292. Ref.:
  17. Josephine FS, Ramya VS, Neelam D, Suresh BG, Siddalingeshwara KG, et al. Isolation, production and characterization of protease from Bacillus sp isolated from soil sample. J Microbiol Biotechnol Res. 2012; 2: 163-168. Ref.: