Three distinct oyster mushroom strains including Pleurotus florida (PF), Pleurotus eous (PE) and Pleurotus sajor-caju (PS) were successfully cultivated on cattail weed substrate. A comparative analysis of different parameters viz., biological efficiency (BE) and protein, carbohydrate, crude fiber and fat content in fruitbodies were evaluated. According to biological efficiencies obtained PF (90%) was superior strain, while order can be represented as 90%> 89%> 82% respectively in PF>PS>PE. The highest protein (4.8 g), lipid (0. 61 g) and dietary fiber (31.6g) values were obtained in the fruitings of Pleurotus sajor-caju. However, the utmost level of carbohydrate (41g) was recorded in Pleurotus eous. On the basis of the observation of the present study we recommend use of cattail weed substrate for cultivation of oyster mushrooms for better nutrients.