The present study was conducted to examine the relationship between work-stress, depression and anxiety among six major professionals (doctors, engineers, bankers, nurses, teachers, and lawyers). The inquiry included 260 professionals from different private and governmental sector organizations of Rawalpindi and Islamabad, Pakistan. Job Stress Scale and the subscales of Depression, Anxiety & Stress Scale for depression and anxiety were administered. Based on the hypotheses, a strong positive correlation was found between work stress and depression & anxiety. The current study revealed that stress at work not only prevailed among different Pakistani professionals on severely alarming levels but significantly contributed in developing depression and anxiety.